ABC 27 first signed on the air in September of 1976 under the call letters WECA. The call letters were the initials of the stations founding owner, E. C. Allen. E. C., as he was known to many, was a local businessman and owner of Mobile Home Industries. Back in those early days the station slogan was "WE CAN do it!", cleverly incorporating the call sign into the slogan. WECA was the third TV station to service the Tallahassee-Thomasville market, following the CBS and PBS stations.
In 1985, E. C. Allen sold the station to Tallahassee 27 Limited Partnership, whose majority partners were Maryland Senator Joseph Tydings and Florida Congressman Lou Frey. It was under this ownership that the call letters were changed to WTXL. The station went through two more ownership changes before its current owners, Southern Broadcast Corporation of Sarasota took over on November 30, 2005.
In 2007, ABC 27 completed a new digital television facility located in Midway, Florida. With this new station, ABC 27 is the first local television station in the Thomasville-Tallahassee market to originate local news in high definition!